TESTING THE TOXICITY OF BIRD'S NEST FERN LEAF EXTRACT (ASPLENIUM NIDUS) ON BRINE SHRIMP LETHALITY TEST BSLT LARVA USING BRINE SHRIMP LETHALITY TEST (BSLT)

Abstract

Background: Bird's nest fern is a plant that lives on other trees and grows a lot in Indonesia. The leaves are the part that is usually used as a mixture of herbal medicines, such as in the treatment of stomach ulcers. This plant contains alkaloids, flavonoids, and steroid terpenoids, saponins. Objectives: The aim of this research was to determine the potential toxicity and LC50 value of bird's nest fern (Asplenium nidus) leaf extract against Artemia salina leach larvae. Method: The research design was experimental using the Brine Shrimp Lethality Test (BSLT) method. Using 150 artemia salina leach larvae and divided into 5 groups with a concentration series of 800 µg/mL, 600 µg/mL, 400 µg/mL, 200 µg/mL. Each group consisted of 10 larvae and 3 replications of treatment. Results: of the research calculated the death of Artemia salina leach larvae for 24 hours and were analyzed using probit analysis to calculate the LC50 value. Namely, the toxicity test shows the LC50 value is 90,99 µg/mL (toxic). It was concluded that the ethanol extract of bird's nest fern leaves has the potential to be toxic to Artemia salina leach larvae with an LC50 value of 106.346 µg/mL.