DETERMINATION OF VITAMIN C CONTENTS FROM ETHANOL EXTRACT OF LIME (Citrus aurantifolia) PEEL USING UV-VIS SPECTROPHOTOMETRY METHOD

Abstract

Baground: Lime peel is a plant that is used as a traditional medicine, the content contained in the skin of lime fruit,  of which is Vitamin C. Vitamin C is a type of natural antioxidant that can ward off harmful free radicals so that it can boost the immune system. Objective: The purpose of this study was to measure the levels of vitamin E in the peel of lime (Citrus aurantifolia) using the UV-Vis spectrophotometer method. Method: The sample used was lime peel. The extraction method used was the maceration method and this type of research was an experiment with direct observation at the laboratory to find out how to find Vitamin C levels in lime peel (Citrus aurantifolia) Determination of vitamin E levels in 10 g lime peel samples was carried out using a spectrophotometer: UV-Vis in the wave range 200-400 nm Determination of the calibration curve was determined from the absorbance value standard solution of vitamin C at concentrations of 5 ppm 10 ppm, 15 ppm 20 ppm and 25 ppm. Result:The results of the research obtained the maximum wavelength of the Bako Vitamin C solution of 266.05 nm and the absorbance of the sample is 79.005. From the determination of the calibration curve, the regress equation is -0.0815x 0.02 with a value equal to 0.09934. Conclusion: From the results of the calculations, it can be concluded that the level of Vitamin C in the peel of lime fruit is 0.0078%.